An integrated scalp and blood biomarker approach suggests the systemic nature of alopecia areata
| Autor: | Dante Dahabreh, James G. Krueger, Giselle Singer, Joseph Han, Celina Dubin, Ning Zhang, Yeriel Estrada, Ester Del Duca, Ana B. Pavel, Jacob W. Glickman, Grace Kimmel, Emma Guttman-Yassky |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Keratins Type II Alopecia Areata Immunology IL1RL1 medicine.disease_cause Lymphocyte Activation Severity of Illness Index Keratins Hair-Specific Immunology and Allergy Medicine Humans CXCL11 Scalp integumentary system business.industry CCL18 Alopecia areata Immune dysregulation medicine.disease Fold change body regions medicine.anatomical_structure Biomarker (medicine) business Biomarkers |
| Zdroj: | AllergyREFERENCES. 76(10) |
| ISSN: | 1398-9995 |
| Popis: | BACKGROUND Alopecia areata (AA) is characterized by immune dysregulation in both scalp and blood, but a large-scale approach establishing biomarkers of AA incorporating both scalp tissue and serum compartments is lacking. We aimed to characterize the transcriptomic signature of AA lesional and nonlesional scalp compared to healthy scalp and determine its relationship with the blood proteome in the same individuals, with comparative correlations to clinical AA disease severity. METHODS We evaluated lesional and nonlesional scalp tissues and serum from patients with moderate-to-severe AA (n = 18) and healthy individuals (n = 8). We assessed 33,118 genes in AA scalp tissue using RNAseq transcriptomic evaluation and 340 inflammatory proteins in serum using OLINK high-throughput proteomics. Univariate and multivariate approaches were used to correlate disease biomarkers with Severity of Alopecia Tool (SALT). RESULTS A total of 608 inflammatory genes were differentially expressed in lesional AA scalp (fold change/FCH>1.5, false discovery rate/FDR |
| Databáze: | OpenAIRE |
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