Optimization of extraction of lipase from wheat seeds (Triticum aestivum) by response surface methodology
| Autor: | J. Vladimir Oliveira, Octavio A. C. Antunes, Morgana Karin Pierozan, Enrique G. Oestreicher, Débora de Oliveira, Rogério Luis Cansian, Roger J. da Costa, Helen Treichel |
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| Rok vydání: | 2009 |
| Předmět: |
Triacylglycerol lipase
Chemical Fractionation Acetone Hydrolysis Response surface methodology Lipase Particle Size Triticum Plant Proteins biology Esterification Precipitation (chemistry) Chemistry Extraction (chemistry) Temperature General Chemistry Hydrogen-Ion Concentration Solvent Biochemistry Seeds biology.protein Particle size General Agricultural and Biological Sciences Nuclear chemistry |
| Zdroj: | Journal of agricultural and food chemistry. 57(20) |
| ISSN: | 1520-5118 |
| Popis: | This work aimed to evaluate the effects of particle size, solid/solvent ratio (w/v), and reaction time on hydrolytic and esterification activities of a lipase extract from wheat seeds. The higher hydrolytic activity was 5.9 U/g with a particle size of 425 microm, solid/solvent ratio of 30:60 (w/v), and reaction time of 15 h, with maximum hydrolytic activity of 14.47 U/g after 24 h of precipitation. For esterification activity the best result was 57.88 U/g with a particle size of 425 microm and solid/solvent ratio of 30:120 (w/v) for 5 h, with a maximum value after 10 h of precipitation reaching 208.20 U/g. The partial characterization showed that the optimal pH and temperature were found to be 5.5 and 32-37 degrees C, respectively. The extract stability at low temperatures was kept after 48 h of storage in terms of esterification activity. The hydrolytic activity was kept constant at -10 degrees C during 72 h and diminished considerably after 24 h at 4 degrees C. |
| Databáze: | OpenAIRE |
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