Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants
Autor: | Mang-Jye Ger, Teng-Yung Feng, Yuh-Chih Tsai, Mei-Kuen Yip, Shih-Hua Chiu, Chin-I Lin, Hsiang-En Huang |
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Rok vydání: | 2006 |
Předmět: |
Zantedeschia
DNA Plant Agrobacterium Blotting Western Plant Science Genetically modified crops Plant disease resistance Polymerase Chain Reaction Microbiology Transformation Genetic Plant Diseases Plant Proteins biology Calla fungi food and beverages General Medicine Plants Genetically Modified biology.organism_classification Blotting Southern Transformation (genetics) Pectobacterium carotovorum Agrobacterium tumefaciens Ferredoxins Agronomy and Crop Science Zantedeschia elliottiana Transformation efficiency |
Zdroj: | Plant Cell Reports. 26:449-457 |
ISSN: | 1432-203X 0721-7714 |
DOI: | 10.1007/s00299-006-0246-y |
Popis: | An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l(-1) kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation. |
Databáze: | OpenAIRE |
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