The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning
| Autor: | Li Ry, Hu Rm, Zhu Zd, Peng Yd, Ze-Guang Han, Yang Ys, Chen Jl, Huang Qh, Huai-Dong Song |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male endocrine system Cancer Research DNA Complementary endocrine system diseases Endocrinology Diabetes and Metabolism Molecular Sequence Data Adrenal Gland Neoplasms Pheochromocytoma Biology medicine.disease_cause Endocrinology Gene expression medicine Humans Amino Acid Sequence RNA Messenger Cloning Molecular Gene Gene Library Expressed Sequence Tags Cloning Expressed sequence tag Base Sequence Adrenal gland Gene Expression Profiling Sequence Analysis DNA medicine.disease Molecular biology Gene expression profiling medicine.anatomical_structure Oncology Carcinogenesis |
| Zdroj: | Endocrine-related cancer. :621-627 |
| ISSN: | 1351-0088 |
| Popis: | Pheochromocytoma is a chromaffin cell neoplasm that typically causes symptoms and signs of episodic catecholamine release. Pheochromocytoma can be divided into two types: familial and sporadic. The molecular mechanisms involved in familial pheochromocytoma have been unraveled, but the detailed molecular mechanism of sporadic pheochromocytoma remains unknown. The present study thus aimed at characterization of gene expression profiling of sporadic pheochromocytoma using expressed sequence tags (ESTs), and established a preliminary catalog of genes expressed in the tumor. In total, 4115 ESTs were generated from the tumor library. The gene expression profilings of the pheochromocytoma and the normal adrenal gland were compared, and 341 genes were identified to be significantly expressed differently between the two libraries. Interestingly, 16 known genes participating in cell division or apoptosis were notably differently expressed between the tumor and the normal adrenal gland. Twenty-four novel full-length cDNAs were cloned from the tumor library and five of them were significantly up-regulated in the tumor. Some of them may be involved in the tumorigenesis of pheochromocytoma. The sequence data of ESTs and novel full-length cDNAs described in this paper have been submitted to the GeneBank library. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|