Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

Autor: Johanne Seguin, Bich-Thuy Doan, Tawba Korichi, Corinne Marie, Hai Doan Do, Nathalie Mignet, Aude Michel, Hélène Dhotel, Christine Ménager
Přispěvatelé: Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Pharmaceutics
Volume 12
Issue 9
Pharmaceutics, MDPI, 2020, 12 (9), pp.854. ⟨10.3390/pharmaceutics12090854⟩
Pharmaceutics, Vol 12, Iss 854, p 854 (2020)
ISSN: 1999-4923
DOI: 10.3390/pharmaceutics12090854
Popis: Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities r2and r1 was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy.
Databáze: OpenAIRE
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