Functional divergence of MYB-related genes, WEREWOLF and AtMYB23 in Arabidopsis
| Autor: | Tomohiko Kato, Satoshi Tabata, Yuka Nukumizu, Rumi Tominaga-Wada, Takuji Wada, Shusei Sato |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Molecular Sequence Data
Arabidopsis Mutant Chimeric Proteins Epidermal cell differentiation Applied Microbiology and Biotechnology Biochemistry Plant Roots Analytical Chemistry Plant Epidermis Evolution Molecular Gene Expression Regulation Plant Arabidopsis thaliana MYB Amino Acid Sequence Molecular Biology Transcription factor Genetics biology Arabidopsis Proteins Organic Chemistry Cell Differentiation General Medicine biology.organism_classification Phenotype Hypocotyl Protein Structure Tertiary DNA-Binding Proteins Plant Leaves Mutation Trichome differentiation Sequence Alignment Functional divergence Biotechnology Transcription Factors |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 76(5) |
| ISSN: | 1347-6947 |
| Popis: | Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased root-hair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|