Xbp1-Independent Ire1 Signaling Is Required for Photoreceptor Differentiation and Rhabdomere Morphogenesis in Drosophila
| Autor: | David Ron, Pedro Domingos, Fátima Cairrão, Ana Varela Coelho, Dina S. Coelho, Xiaomei Zeng, Elisabete Pires, Hyung Don Ryoo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Rhabdomere morphogenesis
XBP1 genetic structures Cellular differentiation Biology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Endoribonucleases Morphogenesis Animals Drosophila Proteins Photoreceptor Cells lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Endoplasmic reticulum Cell Differentiation Rhabdomere Transport protein Cell biology DNA-Binding Proteins lcsh:Biology (General) Unfolded protein response Unfolded Protein Response Drosophila sense organs 030217 neurology & neurosurgery Drosophila Protein Signal Transduction |
| Zdroj: | Cell Reports, Vol 5, Iss 3, Pp 791-801 (2013) |
| ISSN: | 2211-1247 |
| Popis: | The Unfolded Protein Response (UPR) is composed by homeostatic signaling pathways that are activated by excessive protein misfolding in the endoplasmic reticulum (ER). Ire1 signaling is an important mediator of the UPR, leading to the activation of the transcription factor Xbp1. Here, we show that Drosophila Ire1 mutant photoreceptors have defects in the delivery of Rhodopsin-1 to the rhabdomere and in the secretion of Spacemaker/Eyes shut into the inter-rhabdomeral space. However, these defects are not observed in Xbp1 mutant photoreceptors. Ire1 mutant retinas have higher mRNA levels for targets of regulated Ire1-dependent decay (RIDD), including for the fatty acid transport protein (fatp). Importantly, downregulation of fatp by RNA interference rescues the Rhodopsin-1 delivery defects observed in Ire1 mutant photoreceptors. Our results show that the role of Ire1 during photoreceptor differentiation is independent of Xbp1 function and demonstrate the physiological relevance of the RIDD mechanism in this specific paradigm. |
| Databáze: | OpenAIRE |
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