Analysis by UPLC-MS-QTOF and antifungal activity of guava (Psidium guajava L.)
| Autor: | Joara Nályda Pereira Carneiro, Camila Fonseca Bezerra, Rafael Pereira da Cruz, Maciel Horácio Ferreira, Edy Sousa de Brito, Débora Lima Sales, Antonio Judson Targino Machado, Amanda K. Sousa, Thiago Sampaio de Freitas, Henrique Douglas Melo Coutinho, Paulo Riceli Vasconcelos Ribeiro, Antonia Thassya Lucas dos Santos, Maria Karollyna do Nascimento Silva, Maria Flaviana Bezerra Morais-Braga, Janaína Esmeraldo Rocha, Josefa Carolaine Pereira da Silva |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antifungal Agents 030106 microbiology Flavonoid Toxicology Mass Spectrometry Candida tropicalis 03 medical and health sciences Candida krusei medicine Candida albicans IC50 Candida chemistry.chemical_classification Psidium biology Traditional medicine Molecular Structure Plant Extracts General Medicine biology.organism_classification Fungicide chemistry Fluconazole Food Science medicine.drug Chromatography Liquid |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 119 |
| ISSN: | 1873-6351 |
| Popis: | Psidium guajava L. is a plant widely used for food and in folk medicine all over the world. Studies have shown that guava leaves have antifungal properties. In this study, Flavonoid and Tannic fractions were tested to investigate their chemical composition and antifungal potential in vitro.21 compounds in the two fractions, presenting a higher content of phenolic compounds. The antifungal assays were performed against Candida albicans, Candida tropicalis and Candida krusei by microdilution to determine the IC50 and the cell viability curve. Minimal Fungicidal Concentration(MFC) and the inhibitory effects of the association of the fractions with Fluconazole, as well as the assays used to verify any morphological changes were performed in microculture chambers based on the concentrations from the microdilution. The IC50 of the isolated fractions and the fractions associated with each other were calculated, varying from 69.29 to 3444.62 μg/mL and the fractions associated with fluconazole varied from 925.56 to 1.57 μg/mL, it was clear that the association of the natural product with the antifungal presented a synergism. The fractions affected pleomorphism capacity and have a potential antifungal activity as they caused fungal inhibition in isolated use, potentiated the action of Fluconazole, reducing its concentration and impeding morphological transition, one of the virulence factors of the genus. |
| Databáze: | OpenAIRE |
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