Transient desialylation in combination with a novel antithrombin deficiency causing a severe and recurrent thrombosis despite anticoagulation therapy
| Autor: | Maria Luisa Lozano, Javier Corral, Raquel López-Gálvez, José Padilla, Nuria Revilla, Dirk Lefeber, Mara Toderici, María Eugenia de la Morena-Barrio, Vicente Vicente, Antonia Miñano, Ángel García-Avello |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Renal function 030204 cardiovascular system & hematology medicine.disease_cause Article law.invention 03 medical and health sciences 0302 clinical medicine law medicine Humans Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Thrombophilia Thrombolytic Therapy GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) chemistry.chemical_classification Mutation Fibrin Multidisciplinary biology business.industry Antithrombin Anticoagulants Thrombosis Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3] medicine.disease Blood proteins Surgery Pedigree 030104 developmental biology chemistry Transferrin Immunology biology.protein Recombinant DNA Female Blood Coagulation Tests business Neuraminidase medicine.drug |
| Zdroj: | Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid Scientific Reports, 7 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep44556 |
| Popis: | An in-depth focused study of specific cases of patients with recurrent thrombosis may help to identify novel circumstances, genetic and acquired factors contributing to the development of this disorder. The aim of this study was to carry out a detailed and sequential analysis of samples from a patient suffering from early and recurrent venous and arterial thrombosis. We performed thrombophilic tests, biochemical, functional, genetic and glycomic analysis of antithrombin and other plasma proteins. The patient carried a new type I antithrombin mutation (p.Ile218del), whose structural relevance was verified in a recombinant model. Experiments with N-glycosidase F and neuraminidase suggested a nearly full desialylation of plasma proteins, which was confirmed by mass spectrometry analysis of transferrin glycoforms. However, partial desialylation and normal patterns were detected in samples collected at other time-points. Desialylation was noticeable after arterial events and was associated with low antithrombin activity, reduced platelet count and glomerular filtration rate. This is the first description of a global and transient desialylation of plasma proteins associated with thrombosis. The decrease in the strong electronegative charge of terminal glycans may modulate hemostatic protein-protein interactions, which in combination with a strong prothrombotic situation, such as antithrombin deficiency, could increase the risk of thrombosis. |
| Databáze: | OpenAIRE |
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