Immunolocalisation of epidermal growth factor (EGF), EGF receptor and transforming growth factor alpha (TGFα) during murine palatogenesis in vivo and in vitro
| Autor: | J. Garner, M. J. Dixon, M. W. J. Ferguson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Embryology
TGF alpha medicine.medical_specialty Mesenchyme Mice Inbred Strains Biology Mice Organ Culture Techniques Epidermal growth factor Internal medicine medicine Animals Epidermal growth factor receptor Epidermal Growth Factor Palate Embryogenesis Cell Biology Transforming Growth Factor alpha Immunohistochemistry Staining Cell biology ErbB Receptors medicine.anatomical_structure Endocrinology Transforming growth factor beta 3 biology.protein Anatomy Developmental Biology Transforming growth factor |
| Zdroj: | Anatomy and Embryology. 184:83-91 |
| ISSN: | 1432-0568 0340-2061 |
| Popis: | The distribution of epidermal growth factor, the epidermal growth factor receptor and transforming growth factor alpha during murine palatogenesis was investigated immunocytochemically. On embryonic day 12 staining for transforming growth factor alpha was present throughout the palatal mesenchyme, with little in the epithelia. On embryonic day 13 staining increased in the palatal epithelia and in the mesenchyme at the tip of the palate. As the palatal shelves fused together (embryonic day 14.5) intense staining for transforming growth factor alpha was seen in the midline epithelial seam and in the subjacent mesenchyme. On embryonic day 15 there was a generalised increase in palatal epithelial staining; this was most marked in the remnants of the degenerating epithelial seam. Mesenchymal staining was, however, uniform. Whilst palatal staining for epidermal growth factor was sparse, at all stages, staining for its receptor was present throughout the palatal epithelia and mesenchyme. This was most intense in the palatal medial edge epithelia at the time of midline epithelial seam degeneration. The regional and temporal differences in staining for the epidermal growth factor receptor and transforming growth factor alpha suggested that these molecules may play an important role in normal palate development in vivo, particularly in degeneration of the midline epithelial seam. |
| Databáze: | OpenAIRE |
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