Effects of hypoxia on growth factor expression in the rat kidney in vivo
| Autor: | Theresia Ritthaler, Michael Bucher, Peter Sandner, Armin Kurtz, Karl Peter Ittner, Günter A.J. Riegger, Bernhard K. Krämer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Platelet-derived growth factor Gene Expression Endothelial Growth Factors Biology Kidney Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Gene expression medicine Animals RNA Messenger education Growth Substances Hypoxia Erythropoietin Platelet-Derived Growth Factor education.field_of_study Endothelin-3 Lymphokines Endothelin-1 Vascular Endothelial Growth Factors Hypoxia (medical) Oxygen tension Endothelin 3 Rats medicine.anatomical_structure Endocrinology chemistry Nephrology biology.protein Kidney Diseases medicine.symptom Platelet-derived growth factor receptor medicine.drug |
| Zdroj: | Kidney International. 51(2):444-447 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1997.59 |
| Popis: | Effects of hypoxia on renal growth factor expression in the rat kidney. There is accumulating evidence from in vitro studies suggesting that the genes of endothelin-1, PDGF, and VEGF are, like the erythropoietin gene, regulated by oxygen tension and by divalent cations. Hypoxia-induced stimulation of, such as endothelin-1, PDGF or VEGF might be involved in the pathogenesis of acute or chronic renal failure, and in renal "inflammatory" diseases (glomerulonephritis, vasculitis, allograft rejection). Hypoxia (8% O 2 ) for six hours caused a 55-fold/l.6-fold increase of renal erythropoietin/endothelin-1 gene expression, whereas endothelin-3, PDGF-A, PDGF-B, and VEGF gene expression was unchanged. Carbon monoxide (0.1%) treatment for six hours stimulated renal erythropoietin gene expression 140-fold; however, endothelin-1, endothelin-3, PDGF-A, PDGF-B, and VEGF gene expression was not affected. Finally, cobalt treatment (60 mg/kg CoCl 2 ) increased only renal erythropoietin/PDGF-B gene expression 5-fold/l.65-fold. These findings suggest that hypoxia is a rather weak stimulus for renal endothelin-1 gene expression, and that renal PDGF and VEGF gene expression in vivo is not sensitive to tissue hypoxia, in contrast to cell culture experiments. The in vivo regulation of endothelin-1, PDGF, and VEGF differs substantially from that of erythropoietin, suggesting that the basic gene regulatory mechanisms may not be the same. |
| Databáze: | OpenAIRE |
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