Purification of c-phycocyanin from Spirulina fusiformis and its effect on the induction of urokinase-type plasminogen activator from calf pulmonary endothelial cells
| Autor: | Harishkumar Madhyastha, Masugi Maruyama, K.S. Radha, Masahiko Sugiki, Sayuri Omura |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Endothelium
medicine.medical_treatment Molecular Sequence Data Pharmaceutical Science Pulmonary Artery Cyanobacteria Fibrin Cell Line Drug Discovery Phycocyanin Fibrinolysis medicine Animals Amino Acid Sequence Pharmacology Urokinase biology Endothelial Cells Urokinase-Type Plasminogen Activator In vitro medicine.anatomical_structure Complementary and alternative medicine Biochemistry Cell culture Enzyme Induction biology.protein Molecular Medicine Cattle Endothelium Vascular Plasminogen activator medicine.drug |
| Zdroj: | Phytomedicine. 13:564-569 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2005.09.006 |
| Popis: | c-Phycocyanin (c-pc), a blue coloured, fluorescent protein was purified from blue-green alga, Spirulina fusiformis and its effect on fibrinolytic system in vascular endothelial cells was investigated. The c-pc consisted of two subunits, alpha and beta, whose molecular masses were 16 and 17 kDa, respectively. N-terminal sequences of both subunits were well conserved compared with other blue green algal phycobiliproteins. Fibrinolytic activity in the medium conditioned by calf pulmonary arterial endothelial cells was measured by the fibrin plate method. The c-pc increased the fibrinolytic activity in dose- and time-dependent manners. Fibrin zymographic studies indicated that c-pc-induced urokinase-type plasminogen activator in the cells. These in vitro results suggest that c-pc from S. fusiformis is a potent profibrinolytic protein in the vascular endothelial system. |
| Databáze: | OpenAIRE |
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