Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy
Autor: | Ming-gang Wei, Wei Sun, Hao Zhu, Yue Tu, Chun-Qing Li, Chunbo Jiang, Wei-Min Jing |
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Rok vydání: | 2015 |
Předmět: |
Proteinuria
Article Subject biology business.industry Renal cortex Renal function lcsh:Other systems of medicine Triptolide Pharmacology lcsh:RZ201-999 medicine.disease Bioinformatics Nephropathy Podocyte Nephrin chemistry.chemical_compound medicine.anatomical_structure Complementary and alternative medicine chemistry microRNA biology.protein Medicine medicine.symptom business Research Article |
Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015) Evidence-based Complementary and Alternative Medicine : eCAM |
ISSN: | 1741-4288 1741-427X |
DOI: | 10.1155/2015/107814 |
Popis: | Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury.Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels.Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs.Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p. |
Databáze: | OpenAIRE |
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