Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects
| Autor: | Tais Felix Szeles, Luiz Marcelo Sá Malbouisson, Larissa Barbalho, Carlos Roberto Ribeiro de Carvalho, Cristina de Oliveira Massoco, Maria José Carvalho Carmona, José Otávio Costa Auler, Paolo Pelosi |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Lung hyperinflation Physiology medicine.medical_treatment Immunology Biophysics Blood Pressure Biochemistry Collapsed Lung Basal (phylogenetics) Heart Rate medicine Humans Oximetry Continuous positive airway pressure General Pharmacology Toxicology and Pharmaceutics Ventilator-induced lung injury Inflammation Lung Continuous Positive Airway Pressure business.industry General Neuroscience Cell Biology General Medicine Flow Cytometry respiratory tract diseases Cytokine medicine.anatomical_structure Anesthesia Breathing Cytokines Female Inflammation Mediators business Airway |
| Popis: | Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 +/- 1.2 years, BMI: 23.8 +/- 3.6 kg/m(2)). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH(2)O steps up to 20 cmH(2)O every 5 breaths. At CPAP 20 cmH(2)O, an inspiratory pressure of 20 cmH(2)O above CPAP was applied, reaching an airway pressure of 40 cmH(2)O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM. TNF-alpha, IL-1beta, IL-6, IL-8, IL-10, and IL-12 were measured by flow cytometry. Lung hyperinflation significantly increased (P0.05) all measured cytokines (TNF-alpha: 1.2 +/- 3.8 vs 6.4 +/- 8.6 pg/mL; IL-1beta: 4.9 +/- 15.6 vs 22.4 +/- 28.4 pg/mL; IL-6: 1.4 +/- 3.3 vs 6.5 +/- 5.6 pg/mL; IL-8: 13.2 +/- 8.8 vs 33.4 +/- 26.4 pg/mL; IL-10: 3.3 +/- 3.3 vs 7.7 +/- 6.5 pg/mL, and IL-12: 3.1 +/- 7.9 vs 9 +/- 11.4 pg/mL), which returned to basal levels 12 h later. A significant correlation was found between changes in pro- (IL-6) and anti-inflammatory (IL-10) cytokines (r = 0.89, P = 0.004). LHM-induced lung stretching was associated with an early inflammatory response in healthy spontaneously breathing subjects. |
| Databáze: | OpenAIRE |
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