Diversity of European spined loaches (genus Cobitis L.): an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species and hybrid determination
| Autor: | M. A. Culling, Martin Flajšhans, Lukáš Choleva, P. Ivanova, I. Dobrovolov, Věra Šlechtová, Vlastimil Šlechta, Jörg Bohlen, Karel Janko, Petr Ráb, Marie Rábová, Henri Persat, Zdeněk Lajbner, Jan Kotusz |
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| Přispěvatelé: | Laboratoire d'Ecologie des Hydrosystèmes Fluviaux (EHF), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Lyvet, Nathalie |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
0303 health sciences Biogeography Zoology Aquatic Science Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences Genome DNA sequencing [SDE.BE] Environmental Sciences/Biodiversity and Ecology 03 medical and health sciences Genus Cobitis Identification (biology) Restriction fragment length polymorphism [SDE.BE]Environmental Sciences/Biodiversity and Ecology Ecology Evolution Behavior and Systematics ComputingMilieux_MISCELLANEOUS 030304 developmental biology Hybrid |
| Zdroj: | Journal of Fish Biology Journal of Fish Biology, Wiley, 2007, 71 (suppl. C), pp.387-408 |
| ISSN: | 0022-1112 1095-8649 |
| Popis: | Although the unique features of asexual reproduction and hybridization among European spined loaches (genus Cobitis) have recently attracted the attention of conservation biologists, faunists and evolutionary biologists, the research has suffered from uncertain identification of specimens and their genomes because of the extreme morphological similarity of all the species within the hybrid complex. In this article, a Europe-wide study is reported, which was performed on samples collected by several research teams. Several complementary methodologies, such as allozyme analysis, karyotyping, flow cytometry and DNA sequencing allowed us to confirm or reject the existence of all previously reported species and their hybrids as well as to uncover several new hybrid biotypes. The biogeography of all the known biotypes, that is, parental species and hybrid biotypes, has been summarized here and the taxonomic position of two undescribed putative species mentioned in previous publications has been established. New polymerase chain reaction restriction fragment length polymorphism markers for species determination have further been developed and applied, which would allow the unambiguous identification of parental species and their genomes in the known hybrid biotypes within the complex. |
| Databáze: | OpenAIRE |
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