Organ-specific alteration in caspase expression and STK3 proteolysis during the aging process
| Autor: | Jean-Bernard Denault, Mélissa Lessard-Beaudoin, Rona K. Graham, Mélissa Laroche, Amal Loudghi, Erich E. Wanker, Guillaume Grenier, Sean-Patrick Riechers, Marie-Josée Demers |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aging Proteolysis Gene Expression Apoptosis Protein Serine-Threonine Kinases Serine-Threonine Kinase 3 Serine 03 medical and health sciences medicine Animals Threonine Caspase medicine.diagnostic_test biology Kinase General Neuroscience Intrinsic apoptosis Neurodegeneration Brain Neurodegenerative Diseases medicine.disease Cell biology Mice Inbred C57BL 030104 developmental biology Biochemistry Organ Specificity Caspases biology.protein Neurology (clinical) Geriatrics and Gerontology Developmental Biology |
| Zdroj: | Neurobiology of aging. 47 |
| ISSN: | 1558-1497 |
| Popis: | Caspases and their substrates are key mediators of apoptosis and strongly implicated in various physiological processes. As the serine/threonine kinase family is involved in apoptosis and serine/threonine kinase 3 (STK3) is a recently identified caspase-6 substrate, we assessed the expression and cleavage of STK3 in murine peripheral organs and brain regions during the aging process. We also assessed caspase-3, -6, -7, and -8 expression and activity in order to delineate potential mechanism(s) underlying the generation of the STK3 fragments observed and their relation to the apoptotic pathway. We demonstrate for the first time the cleavage of STK3 by caspase-7 and show that STK3 protein levels globally increase throughout the organism with age. In contrast, caspase-3, -6, -7, and -8 expression and activity vary significantly among the different organs analyzed suggesting differential effects of aging on the apoptotic mechanism and/or nonapoptotic functions of caspases throughout the organism. These results further our understanding of the role of caspases and their substrates in the normal aging process and highlight a potential role for STK3 in neurodegeneration. |
| Databáze: | OpenAIRE |
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