Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study
| Autor: | Guillermo J. Tearney, Jason R. McCarthy, Farouc A. Jaffer, Scott A. Hilderbrand, Charles P. Lin, S. Sibel Erdem, Michael R. Jaff, Ashley F. Stein-Merlob, Henry Zelada, Guy L. Reed, Peter K. Henke, Chase W. Kessinger |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Indoles pulmonary embolism medicine.medical_treatment Medicine (miscellaneous) 030204 cardiovascular system & hematology Tissue plasminogen activator Fibrin 03 medical and health sciences 0302 clinical medicine Fibrinolytic Agents Fibrinolysis medicine Animals Thrombus Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology Venous Thrombosis 0303 health sciences biology Staining and Labeling business.industry Thrombosis medicine.disease molecular imaging 3. Good health Mice Inbred C57BL Venous thrombosis Disease Models Animal biology.protein fibrinolysis business Oligopeptides Fibrinolytic agent Intravital microscopy medicine.drug Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | Fibrinolytic therapy of venous thromboembolism (VTE) is increasingly utilized, yet limited knowledge is available regarding in vivo mechanisms that govern fibrinolytic efficacy. In particular, it is unknown how age-dependent thrombus organization limits direct blood contact with fibrin, the target of blood-based fibrinolytic agents. Utilizing high-resolution in vivo optical molecular imaging with FTP11, a near-infrared fluorescence (NIRF) fibrin-specific reporter, here we investigated the in vivo interrelationships of blood accessibility to fibrin, thrombus age, thrombus neoendothelialization, and fibrinolysis in murine venous thrombosis (VT). In both stasis VT and non-stasis VT, NIRF microscopy showed that FTP11 fibrin binding was thrombus age-dependent. FTP11 localized to the luminal surface of early-stage VT, but only minimally to subacute VT (p |
| Databáze: | OpenAIRE |
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