Double-stranded RNA induces disproportionate expression of thymic stromal lymphopoietin versus interferon- in bronchial epithelial cells from donors with asthma
| Autor: | Peter H. Howarth, Ben Green, Lena Uller, Nicole Bedke, Stephen T. Holgate, Donna E. Davies, Laurie Lau, Marina S. Leino, David Sammut |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Thymic stromal lymphopoietin medicine.medical_treatment Dose-Response Relationship Immunologic Bronchi Respiratory Mucosa Allergic inflammation Young Adult Immune system Thymic Stromal Lymphopoietin Interferon Bronchoscopy Humans Medicine RNA Messenger Interleukin 8 Protein Kinase Inhibitors Cells Cultured RNA Double-Stranded business.industry Interleukin-8 Interleukin Epithelial Cells Interferon-beta Middle Aged Acquired immune system Asthma Immunity Innate Toll-Like Receptor 3 Cytokine Gene Expression Regulation Immunology Cytokines Female business medicine.drug |
| Zdroj: | Thorax. 65:626-632 |
| ISSN: | 0040-6376 |
| Popis: | Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine that strongly activates dendritic cells and can initiate allergic inflammation. Since exposure to rhinovirus or double-stranded (ds) RNA (a surrogate of viral infection) induces TSLP expression in bronchial epithelial cells (BECs), this cytokine may link innate antiviral responses and the type 2 adaptive immune response.As BECs from donors with asthma have a deficient interferon (IFN) response to rhinovirus infection, a study was undertaken to test the hypothesis that their antiviral response shows a bias towards TSLP production.Primary BECs were grown from subjects with asthma and healthy volunteers. After exposure to dsRNA, interleukin (IL)-8, IFNbeta and TSLP mRNA and protein expression were measured by RT-qPCR and ELISA, respectively.dsRNA dose-dependently increased IL-8 expression in BECs with no significant difference between the groups. However, BECs from subjects with asthma expressed less IFNbeta and more TSLP mRNA and protein in response to dsRNA than BECs from those without asthma (median (IQR) 57 (38-82) pg/ml vs 106 (57-214) pg/ml for IFNbeta (p0.05) and 114 (86-143) pg/ml vs 65 (32-119) pg/ml for TSLP (p0.05) in response to 10 microg/ml dsRNA for 24 h). Induction of TSLP mRNA by dsRNA was blocked by Toll-like receptor 3 or protein kinase inhibitors or by preventing de novo protein synthesis, but not by neutralisation of type I IFN receptors.BECs from subjects with asthma are biased towards higher TSLP and lower IFNbeta production in response to dsRNA, suggesting that viral infection in asthma may lead to an altered mediator profile that biases towards a Th2 immune response. |
| Databáze: | OpenAIRE |
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