Preparation, antiangiogenic and antitumoral activities of the chemically sulfated glucan from Phellinus ribis
| Autor: | Peng George Wang, Haiqiang Jiang, Yuguo Liu, Yuhong Liu, Lingchuan Xu, Fengshan Wang, Yan-Na Cheng |
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| Rok vydání: | 2014 |
| Předmět: |
Carcinoma
Hepatocellular beta-Glucans Polymers and Plastics Molecular Sequence Data Angiogenesis Inhibitors Antineoplastic Agents Umbilical vein Immunoenzyme Techniques Mice chemistry.chemical_compound Sulfation In vivo Human Umbilical Vein Endothelial Cells Materials Chemistry Animals Humans Cytotoxic T cell Glucans Cells Cultured Zebrafish Cell Proliferation Glucan Ovarian Neoplasms chemistry.chemical_classification Mice Inbred BALB C Neovascularization Pathologic Basidiomycota Liver Neoplasms Organic Chemistry Xenograft Model Antitumor Assays Molecular biology In vitro Vascular endothelial growth factor Carbohydrate Sequence chemistry Biochemistry Toxicity Female |
| Zdroj: | Carbohydrate Polymers. 106:42-48 |
| ISSN: | 0144-8617 |
| Popis: | Two sulfated derivatives (PRP-S1 and PRP-S2) of a β-glucan from Phellinus ribis with different degrees of substitution were obtained by chlorosulfonic acid method. The derivatives could block formation of new vessels in zebrafish and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs). The two sulfated derivatives had remarkably high antitumor activities in vivo (in BALB/c mice inoculated with H22 hepatocellular carcinoma) as well as in vitro (against human ovary cancer SKOV-3 cells), without producing any overt signs of general toxicity. The results of immunohistochemistry assay indicated that the derivatives significantly reduced the average number of microvessel density (MVD) and inhibited the expression of vascular endothelial growth factor (VEGF) in tumor. Thus, these derivatives exhibit pronounced antiangiogenic and antitumoral properties. Except for cytotoxic effects on tumor cells, it is reasonable to expect that the antitumoral effects of PRP-S1 and PRP-S2 are mediated via their antiangiogenic properties. |
| Databáze: | OpenAIRE |
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