Covalent Immobilization of Cellulase Using Magnetic Poly(ionic liquid) Support: Improvement of the Enzyme Activity and Stability
| Autor: | Mahshid Yaghoubi, Nasrin Zohreh, Seyedeh Ameneh Hosseini, Seyed Hassan Hosseini, Ali Pourjavadi |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Hot Temperature Polymers Ionic Liquids Ionic bonding 02 engineering and technology Cellulase Ferric Compounds 01 natural sciences chemistry.chemical_compound Adsorption 010608 biotechnology Enzyme Stability Thermal stability Magnetite Nanoparticles Nanocomposite biology Magnetic Phenomena General Chemistry Enzymes Immobilized 021001 nanoscience & nanotechnology Monomer chemistry Chemical engineering Covalent bond Ionic liquid biology.protein 0210 nano-technology General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:789-798 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b03922 |
| Popis: | A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase. |
| Databáze: | OpenAIRE |
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