Multidrug resistance and retroviral transduction potential in human small cell lung cancer cell lines
| Autor: | Gregers J. Gram, Peter Buhl Jensen, Mette Due Theilade, Maurizio Cianfriglia, John-Erik Stig Hansen, Mikael Rørth |
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| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Lung Neoplasms Genetic enhancement Genetic Vectors Virus Pathology and Forensic Medicine Transduction (genetics) Murine leukemia virus Tumor Cells Cultured Humans Immunology and Allergy ATP Binding Cassette Transporter Subfamily B Member 1 Carcinoma Small Cell Receptor biology Genetic transfer Genetic Therapy General Medicine Cell Transformation Viral Flow Cytometry biology.organism_classification Drug Resistance Multiple Clone Cells respiratory tract diseases Multiple drug resistance Retroviridae Microscopy Fluorescence Cell culture Immunology Cancer research Receptors Virus |
| Zdroj: | APMIS. 107:851-858 |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1111/j.1699-0463.1999.tb01482.x |
| Popis: | Multidrug resistance (MDR) remains a major problem in the successful treatment of small cell lung cancer (SCLC). New treatment strategies are needed, such as gene therapy specifically targeting the MDR cells in the tumor. Retroviral LacZ gene-containing vectors that were either pseudotyped for the gibbon ape leukemia virus (GALV-1) receptor or had specificity for the amphotropic murine leukemia virus (MLV-A) receptor were used for transduction of five SCLC cell lines differing by a range of MDR mechanisms. Transduction efficiencies in these cell lines were compared by calculating the percentage of blue colonies after X-Gal staining of the cells grown in soft agar. All examined SCLC cell lines were transducible with either vector. Transduction efficiencies varied from 5.7% to 33.5% independent of the presence of MDR. These results indicate that MDR does not severely impair transduction of SCLC cells, and that MLV-A as well as GALV-1 retroviral vectors are suitable for further development of gene therapy in SCLC. |
| Databáze: | OpenAIRE |
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