The structural and molecular analysis of endoreduplicated nuclei in tomato (Solanum lycopersicum) fruit provides evidence for a ploidy-dependent increase in transcriptional activity
| Autor: | Jean-Pierre Renaudin, Olivier Coriton, Catherine Cheniclet, Spencer Brown, Nathalie Frangne, Mickael Bourge, Matthieu Bourdon, Christian Chevalier, Julien Pirrello |
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| Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, La plante et son environnement (PSE), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
kinase growth plant Plant Science In situ hybridization Biology 01 natural sciences endoreduplication 03 medical and health sciences tomate physiologie végétale plante fruitière Polyploid Endoreduplication [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Mitosis Gene genome 030304 developmental biology Genetics fish 0303 health sciences Polytene chromosome Vegetal Biology karyoplasmic homeostasis food and beverages fruit anaphase promoting complex Chromatin Cell biology cell size arabidopsis solanum lycopersicum développement du fruit tomato fruit transcription bac fish Ploidy Agronomy and Crop Science Biologie végétale 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology (Tsukuba) 3 (30), 301-307. (2013) Plant Biotechnology Plant Biotechnology, 2013, 30 (3), pp.301-307. ⟨10.5511/plantbiotechnology.13.0319b⟩ Plant Biotechnology, Japanese Society for Plant Cell and Molecular Biology, 2013, 30 (3), pp.301-307. ⟨10.5511/plantbiotechnology.13.0319b⟩ |
| ISSN: | 1342-4580 1347-6114 |
| DOI: | 10.5511/plantbiotechnology.13.0319b⟩ |
| Popis: | International audience; Endopolyploidy, i.e. the amplification of genomic DNA without mitosis, is a widespread process in plants. Cells from the tomato fruit pericarp are characterized by a wide range of ploidy levels (from 2C to 256C). Although various functional hypotheses have been attributed to endoreduplication according to the literature, evidence for a specific role of endoreduplication in transcription and metabolism control is still lacking. We have developed a new method based on bacterial artificial chromosome fluorescent in situ hybridization (BAC-FISH) that allows the in situ determination of DNA ploidy levels of individual nuclei. The advantage of this method is illustrated by the analysis of ploidy levels and cell sizes within the pericarp tissue from mature green tomato fruits. Using this cellular approach we established the ploidy map of the pericarp tissue. Based on this map, we performed a structural analysis of endoreduplicated nuclei at the level of chromatin organization, nuclear shape and relationship with mitochondria. We demonstrated a link between the ploidy level of nuclei, the complexity of their shape and the number of mitochondria at the vicinity of polyploid nuclei. The use of the DNA FISH method demonstrated that endopolyploidy leads to the formation of polytene chromosomes, whereas the use of a RNA FISH method demonstrated that the rDNA transcription was increased during polyploidization. Performing quantitative PCR (qPCR) and RT-qPCR on sorted nuclei respectively, we confirmed that endoreduplication did amplified exponentially loci for a set of specific genes allowing us to demonstrade that endoreduplication results in an increasing transcriptional activity. |
| Databáze: | OpenAIRE |
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