Pro-renin receptor suppresses mitochondrial biogenesis and function via AMPK/SIRT-1/ PGC-1α pathway in diabetic kidney

Autor:	Safia Akhtar, Helmy M. Siragy
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Male Biopsy Protein Expression Gene Expression Mitochondrion AMP-Activated Protein Kinases Biochemistry Mice 0302 clinical medicine Endocrinology Adenosine Triphosphate Sirtuin 1 Gene expression Medicine and Health Sciences Small interfering RNAs Diabetic Nephropathies Prorenin Receptor Receptor Energy-Producing Organelles Gene knockdown Multidisciplinary Chemistry Organic Compounds Monosaccharides Immunohistochemistry Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Mitochondrial DNA 3. Good health Mitochondria Nucleic acids Physical Sciences Mesangial Cells Medicine RNA Interference Signal transduction Cellular Structures and Organelles Anatomy Research Article Signal Transduction medicine.medical_specialty DNA Copy Number Variations Endocrine Disorders Forms of DNA Science Carbohydrates NF-E2-Related Factor 1 030209 endocrinology & metabolism Receptors Cell Surface Bioenergetics Biosynthesis Research and Analysis Methods Cell Line Diabetes Mellitus Experimental 03 medical and health sciences Internal medicine medicine Diabetes Mellitus Genetics Gene Expression and Vector Techniques Animals Non-coding RNA Molecular Biology Techniques Molecular Biology Molecular Biology Assays and Analysis Techniques Organic Chemistry Chemical Compounds AMPK Biology and Life Sciences Kidneys Cell Biology Renal System DNA TFAM Gene regulation Disease Models Animal 030104 developmental biology Glucose Mitochondrial biogenesis Metabolic Disorders RNA Biomarkers
Zdroj:	PLoS ONE PLoS ONE, Vol 14, Iss 12, p e0225728 (2019)
ISSN:	1932-6203
Popis:	Abnormal mitochondrial biogenesis and function has been linked to multiple diseases including diabetes. Recently, we demonstrated the role of renal (Pro)renin receptor (PRR) in the dysregulation of mitochondria. We hypothesized that PRR contributes to the reduction of mitochondrial biogenesis and function in diabetic kidney via PGC-1α/AMPK/SIRT-1 signaling pathway. In vivo and in vitro studies were conducted in C57BL/6 mouse and mouse renal mesangial cells (mRMCs). Control and streptozotocin-induced diabetic mice were injected with scramble or PRR shRNA and followed for a period of eight weeks. PRR mRNA and protein expression increased by 44% and 39% respectively (P
Databáze:	OpenAIRE
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