Recombinant DNA/MVA/ChAdV-63-elicited T cells specific for conserved regions of the HIV-1 proteome recognize HIV-1 infected cells and suppress HIV-1
| Autor: | Nicola Borthwick, L Yorke, T Ahmed, Andrew J. McMichael, Lucy Dorrell, Hongbing Yang, Tomáš Hanke, A Rose, A P Black, U Ebrahimsa, Emma-Jo Hayton, Gemma Hancock |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Chemokine biology medicine.diagnostic_test ELISPOT T cell virus diseases Bioinformatics Virology Flow cytometry medicine.anatomical_structure Multiplicity of infection Infectious Diseases Viral replication Poster Presentation biology.protein medicine Antibody lcsh:RC581-607 CD8 |
| Zdroj: | Retrovirology Retrovirology, Vol 9, Iss Suppl 2, p P259 (2012) |
| ISSN: | 1742-4690 |
| Popis: | Results HIV-1 infection kinetics is influenced by the multiplicity of infection (MOI) used to infect autologous CD4+ cells, with rapid kinetics observed at higher MOIs. For HIV-1 BaL optimal infection of autologous CD4+ cells was achieved at MOI of 0.05. In the VSA, HIV-1 BaL virus replication was shown to be sensitive to the chemokines MIP-1a and RANTES added in the absence of effector cells. Pre-activated effector cells indicated increased nonspecific background suppression in healthy controls, which was reduced following a prolonged rest before coculture with autologous CD4+ targets, but led to marked proliferation of the CD8+ T cell effectors. Preliminary investigations in vaccinees show that HIV-1 suppression mediated by CD8+ T cells can be detected in vitro following vaccination and that P24 ELISA has higher sensitivity than flow cytometry. As an alternative to the VSA, an IFN-g ELISpot assay has also been optimized for the use of autologous HIV-1-infected CD4+ cells. Using both of the above assays, preliminary characterization of T cell responses induced in volunteers receiving pSG2.HIVconsv DNA, MVA.HIVconsv and ChAdV63.HIVconsv vaccines will be shown. |
| Databáze: | OpenAIRE |
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