Complex Regulation by Apetala2 Domain-Containing Transcription Factors Revealed through Analysis of the Stress-Responsive TdCor410b Promoter from Durum Wheat
| Autor: | Natalia Tikhomirov, Katherine A. Pillman, Omid Eini, Sergiy Lopato, Scott V. Tingey, Neil J. Shirley, Natalia Bazanova, Nannan Yang, Shoba Sivasankar, Serik Eliby, Tatiana Pyvovarenko, Peter Langridge, Maria Hrmova |
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| Přispěvatelé: | Eini, Omid, Yang, Nannan, Pyvovarenko, Tatiana, Pillman, Katherine, Bazanova, Natalia, Tikhomirov, Natalia, Eliby, Serik, Shirley, Neil, Sivasankar, Shoba, Tingey, Scott, Langridge, Peter, Hrmova, Maria, Lopato, Sergiy |
| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular 0106 biological sciences Subfamily Agricultural Biotechnology Response element lcsh:Medicine plant Plant Science phylogeny Plant Genetics Biochemistry 01 natural sciences Computational Chemistry Gene Expression Regulation Plant Molecular Cell Biology Gene expression Biochemical Simulations Biomacromolecule-Ligand Interactions genes lcsh:Science Biochemistry Simulations Promoter Regions Genetic Phylogeny Triticum Plant Proteins 2. Zero hunger Regulation of gene expression Genetics 0303 health sciences Multidisciplinary Physics food and beverages genetically modified Agriculture gene expression regulation Plants Genetically Modified Chemistry Wheat plant proteins Research Article DNA Plant Molecular Sequence Data Biophysics Cereals molecular sequence data Crops Sequence alignment Biology Genes Plant 03 medical and health sciences Stress Physiological transcription factors Amino Acid Sequence Gene Transcription factor 030304 developmental biology Base Sequence Sequence Homology Amino Acid triticum cDNA library lcsh:R fungi Computational Biology DNA sequence homology base sequence amino acid sequence lcsh:Q Transcription Factors 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 3, p e58713 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0058713 |
| Popis: | Expression of the wheat dehydrin gene Cor410b is induced several fold above its non-stressed levels upon exposure to stresses such as cold, drought and wounding. Deletion analysis of the TdCor410b promoter revealed a single functional C-repeat (CRT) element. Seven transcription factors (TFs) were shown to bind to this CRT element using yeast one-hybrid screens of wheat and barley cDNA libraries, of which only one belonged to the DREB class of TFs. The remaining six encoded ethylene response factors (ERFs) belong to three separate subfamilies. Analysis of binding selectivity of these TFs indicated that all seven could bind to the CRT element (GCCGAC), and that three of the six ERFs could bind both to the CRT element and the ethylene-responsive GCC-box (GCCGCC). The TaERF4 subfamily members specifically bound the CRT element, and did not bind either the GCC-box or DRE element (ACCGAC). Molecular modeling and site-directed mutagenesis identified a single residue Pro42 in the Apetala2 (AP2) domain of TaERF4-like proteins that is conserved in monocotyledonous plants and is responsible for the recognition selectivity of this subfamily. We suggest that both DREB and ERF proteins regulate expression of the Cor410b gene through a single, critical CRT element. Members of the TaERF4 subfamily are specific, positive regulators of Cor410b gene expression. |
| Databáze: | OpenAIRE |
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