The histone variant H2A.W and linker histone H1 co-regulate heterochromatin accessibility and DNA methylation

Autor: Suhua Feng, Olivier Mathieu, Pierre Bourguet, Anna Schmücker, Steven E. Jacobsen, Thierry Pélissier, Frédéric Berger, Marie-Noëlle Pouch-Pélissier, Colette L. Picard, Ramesh Yelagandula, Zdravko J. Lorković
Přispěvatelé: Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), University of California [Los Angeles] (UCLA), University of California, Austrian Academy of Sciences (OeAW), Vienna Biocenter - VBC [Austria], University of California (UC), Mathieu, Olivier
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
animal structures
DNA
Plant
Heterochromatin
Science
Arabidopsis
General Physics and Astronomy
[SDV.GEN] Life Sciences [q-bio]/Genetics
General Biochemistry
Genetics and Molecular Biology
Histones
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
0302 clinical medicine
Histone H1
Gene Expression Regulation
Plant
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Epigenetics
Heterochromatin organization
030304 developmental biology
0303 health sciences
[SDV.GEN]Life Sciences [q-bio]/Genetics
Multidisciplinary
Whole Genome Sequencing
biology
Arabidopsis Proteins
Chemistry
fungi
Genetic Variation
Nuclear Proteins
General Chemistry
Methylation
DNA Methylation
Chromatin
Nucleosomes
Cell biology
Plant Leaves
030104 developmental biology
Histone
Mutation
DNA methylation
embryonic structures
biology.protein
[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
030217 neurology & neurosurgery
Zdroj: Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-22993-5⟩
Nature Communications, Nature Publishing Group, 2021, ⟨10.1038/s41467-021-22993-5⟩
Nature Communications, 2021, ⟨10.1038/s41467-021-22993-5⟩
Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-021-22993-5⟩
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
ISSN: 2041-1723
Popis: In flowering plants, heterochromatin is demarcated by the histone variant H2A.W, elevated levels of the linker histone H1, and specific epigenetic modifications, such as high levels of DNA methylation at both CG and non-CG sites. How H2A.W regulates heterochromatin organization and interacts with other heterochromatic features is unclear. To analyze the in vivo function of H2A.W, we created a h2a.w null mutant via CRISPR-Cas9, h2a.w-2. We find that H2A.W antagonizes deposition of H1 at heterochromatin and that non-CG methylation and accessibility are moderately decreased in h2a.w-2 heterochromatin. Compared to H1 loss alone, combined loss of H1 and H2A.W greatly increases accessibility and facilitates non-CG DNA methylation in heterochromatin, suggesting co-regulation of heterochromatic features by H2A.W and H1. Our results suggest that H2A.W helps maintain optimal heterochromatin accessibility and DNA methylation by promoting chromatin compaction together with H1, while also inhibiting excessive H1 incorporation.
Databáze: OpenAIRE
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