Optimisation by experimental design of a capillary electrophoretic method for the separation of several inhibitors of angiotensin-converting enzyme using alkylsulphonates
| Autor: | W. Van den Bossche, Y. Vander Heyden, S Hillaert |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Ramipril
Chromatography Chemistry Organic Chemistry Lisinopril Electrophoresis Capillary Angiotensin-Converting Enzyme Inhibitors General Medicine Factorial experiment Hydrogen-Ion Concentration Reference Standards Cilazapril Biochemistry Analytical Chemistry Chemometrics Capillary electrophoresis Quinapril Fosinopril medicine Regression Analysis Sulfonic Acids medicine.drug |
| Zdroj: | Journal of Chromatography A. 978:231-242 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(02)01388-2 |
| Popis: | A statistical experimental design was used to optimise a capillary electrophoretic separation method for eight inhibitors of the angiotensin-converting enzyme: enalapril, lisinopril, quinapril, fosinopril, perindopril, ramipril, benazepril, and cilazapril. Because a free solution capillary electrophoresis system did not achieve a complete separation of these eight compounds in one run, the usefulness of alkylsulphonates as ion-pairing agents was investigated. After preliminary investigations to determine the experimental domain and the most important factors, a three-level full-factorial design was applied to study the impact of the pH and the molarity of the ion-pairing agent on the separation. Improved separations were obtained suggesting a favourable effect of ion-pairing interactions between analytes and the additive; however, it remained impossible to separate them all in one run. A combination of two systems was still necessary for the selective identification of these structurally-related substances. |
| Databáze: | OpenAIRE |
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