Antioxidant and cytotoxic activities of three species of tropical seaweeds
| Autor: | Wai Sum Yap, Kong Soo Khoo, Yin Yin Chia, M.S. Kanthimathi, Hwee Ming Cheng, Jayakumar Rajarajeswaran |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Apoptosis DNA fragmentation Phaeophyta Antioxidants Superoxide dismutase Caulerpa racemosa chemistry.chemical_compound Antioxidant activity Turbinaria ornata medicine Humans MTT assay Antihypertensive Agents Cytotoxic activity biology Plant Extracts Superoxide Dismutase Padina tetrastromatica Antioxidant enzyme activity General Medicine Catalase Seaweed biology.organism_classification LC-MS chemistry Biochemistry Complementary and alternative medicine MCF-7 Cells biology.protein Caspase activity Oxidation-Reduction Camptothecin Research Article medicine.drug |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-015-0867-1 |
| Popis: | Background Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified. Methods Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation. Results All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine. Conclusion Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits. |
| Databáze: | OpenAIRE |
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