Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens
| Autor: | Karen C. Carroll, Kim Dionne, Nicole Parrish, Annie Hedgepeth, Cindy Hendry |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Time Factors Tuberculosis Sensitivity and Specificity Fluorescence Mycobacterium Microbiology chemistry.chemical_compound medicine Humans Rapid stain Fluorescent Dyes Staining and Labeling biology Auramine O Differential staining Mycobacteriology and Aerobic Actinomycetes biology.organism_classification medicine.disease Molecular biology Staining chemistry Benzophenoneidum Fluorescent staining Bacteria |
| Zdroj: | Journal of Clinical Microbiology. 47:1206-1208 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time (∼2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories. |
| Databáze: | OpenAIRE |
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