Vasopressin Gene Expression in Hypertensive, Normotensive, and Diabetes Insipidus Rats
| Autor: | K. Almasan, R. E. Lang, Jörg Hänze, Joseph T. McCabe, Detlev Ganten, Donald W. Pfaff, Elke Lehmann |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
medicine.medical_specialty Vasopressin Transcription Genetic Vasopressins In situ hybridization Rats Inbred WKY Exon Spontaneously hypertensive rat Rats Inbred SHR Internal medicine Gene expression Internal Medicine medicine Animals RNA Messenger Messenger RNA biology Rats Brattleboro biology.organism_classification medicine.disease Brattleboro rat Rats Endocrinology Hypertension Diabetes insipidus Diabetes Insipidus hormones hormone substitutes and hormone antagonists |
| Zdroj: | Clinical and Experimental Hypertension. Part A: Theory and Practice. 10:131-142 |
| ISSN: | 0730-0077 |
| DOI: | 10.3109/10641968809075968 |
| Popis: | This investigation examined the presence and abundance of vasopressin-gene messenger ribonucleic acid (mRNA) transcripts in hypothalamic tissue from five strains of rats: Long Evans, Wistar-Kyoto, and diabetes insipidus (Brattleboro) rats, stroke-prone spontaneously hypertensive rats, and cross-bred diabetes insipidus x stroke-prone spontaneously hypertensive rats. A single-stranded RNA probe complementary to exon C of the vasopressin gene was utilized for in situ hybridization and identified hypothalamic 'vasopressinergic' neurons in tissue from all five strains of rats. The results obtained by solution and in situ hybridization suggested the cross-bred diabetic-hypertensive rat exhibits a level of vasopressin-gene messenger ribonucleic acid similar to diabetes insipidus rats. This observation is consistent with previous physiological data which suggests cross-bred diabetic-hypertensive rats inherit the mutated vasopressin gene of the Brattleboro rat. |
| Databáze: | OpenAIRE |
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