Possible role of microRNA miRNA-IL-25 interaction in mice with ulcerative colitis
| Autor: | Jian-yao Wang, Ruoyu Gao, Jun Yao, Ben-Hua Wu, Li-Sheng Wang, Ming-Han Luo, Cheng Wei, De-Feng Li, Liliangzi Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) microrna Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Bioengineering Biology Applied Microbiology and Biotechnology mir-691 Mice 03 medical and health sciences 0302 clinical medicine microRNA medicine Animals RNA Messenger ulcerative colitis il-25 Sequence Analysis RNA Interleukins General Medicine medicine.disease Ulcerative colitis mir-135b-5p Mice Inbred C57BL MicroRNAs 030104 developmental biology Immunology Colitis Ulcerative 030211 gastroenterology & hepatology TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered, Vol 11, Iss 1, Pp 862-871 (2020) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | Background: The regulatory network of ulcerative colitis (UC)-associated miRNAs is not fully understood. In this study, we aim to investigate the global profile and regulatory network of UC associated miRNAs in the context of dextran sulfate sodium (DSS). Methods: UC was induced in C57BL mice using DSS. Differentially expressed miRNAs were screened by RNA sequencing and subjected to the Kyoto Encyclopedia of Genes and Genomes Pathway enrichment analysis. RT-qPCR was used to verify the differential expression of miRNAs and candidate target mRNA. Luciferase reporter vector bearing a miRNA binding site was constructed to verify the binding site of the miRNA on mRNA. Results:A total of 95 miRNAs (31 were up-regulated and 64 were down regulated) differentially expressed in the colonic tissues of the UC mice. Among the differentially expressed miRNAs, IL-25 pathway genes were enriched. Subsequent RT-qPCR confirmed a decreased expression of IL-25 and a significant up regulation of IL-25 target miRNAs including mmu-miR-135b-5p, mmu-miR-7239-5p and mmu-miR-691 in UC mice. Conclusion: Using the luciferase assay, we verified the biding sites of mmu-miR-135b-5p and mmu-miR-691 to the IL-25 3ʹUTR. In conclusion, mmu-miR-135b-5p:IL-25 and mmu-miR-691:IL-25 interactions are important pathways that may exert a protective role in UC. Graphical abstract |
| Databáze: | OpenAIRE |
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