Dual effects of interleukin-1beta on N-methyl-D-aspartate-induced retinal neuronal death in rat eyes

Autor: Shinji Yoneda, Hidenobu Tanihara, Nobuaki Miyawaki, Masaru Inatani, Yoshihito Honda, Megumi Honjo, Noriaki Kido, Hideaki Hara
Rok vydání: 2001
Předmět:
Male
medicine.medical_specialty
N-Methylaspartate
Eye Diseases
medicine.drug_class
Sialoglycoproteins
Neurotoxins
Excitotoxicity
Glutamic Acid
Biology
medicine.disease_cause
Receptors
N-Methyl-D-Aspartate

Retina
Rats
Sprague-Dawley

chemistry.chemical_compound
Internal medicine
medicine
Excitatory Amino Acid Agonists
Animals
Drug Interactions
Molecular Biology
Neurons
Cell Death
Dose-Response Relationship
Drug

General Neuroscience
Retinal
Receptor antagonist
Inner plexiform layer
Immunohistochemistry
Rats
Up-Regulation
Drug Combinations
Interleukin 1 Receptor Antagonist Protein
Endocrinology
medicine.anatomical_structure
Neuroprotective Agents
chemistry
Retinal ganglion cell
Nerve Degeneration
NMDA receptor
sense organs
Neurology (clinical)
Halothane
Developmental Biology
medicine.drug
Interleukin-1
Zdroj: Brain research. 910(1-2)
ISSN: 0006-8993
Popis: In this study we determine if interleukin-1beta (IL-1beta) modulates N-methyl-D-aspartate (NMDA)-induced retinal damage. Sprague-Dawley rats were anesthetized with inhalation of halothane, after which a single injection of 5 microl of IL-1beta (0.1 to 10 ng/eye) (and/or IL-1 receptor antagonist (IL-1ra)) for experimental eyes was administered. Two days later (or simultaneously), NMDA (20 nmol) was injected into the vitreous space. One week later, each eye was enucleated and transverse sections were subjected to morphometric analysis. Enzyme-linked immunosorbent assay (ELISA) was conducted for the determination of IL-1beta levels in retina. Immunohistochemical and immunoblot studies were also performed. In eyes that received an intravitreal injection of IL-1beta (0.1 to 10 ng/eye), significant thinning of the inner plexiform layer (IPL) was observed (P
Databáze: OpenAIRE