Dual effects of interleukin-1beta on N-methyl-D-aspartate-induced retinal neuronal death in rat eyes
Autor: | Shinji Yoneda, Hidenobu Tanihara, Nobuaki Miyawaki, Masaru Inatani, Yoshihito Honda, Megumi Honjo, Noriaki Kido, Hideaki Hara |
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Rok vydání: | 2001 |
Předmět: |
Male
medicine.medical_specialty N-Methylaspartate Eye Diseases medicine.drug_class Sialoglycoproteins Neurotoxins Excitotoxicity Glutamic Acid Biology medicine.disease_cause Receptors N-Methyl-D-Aspartate Retina Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Excitatory Amino Acid Agonists Animals Drug Interactions Molecular Biology Neurons Cell Death Dose-Response Relationship Drug General Neuroscience Retinal Receptor antagonist Inner plexiform layer Immunohistochemistry Rats Up-Regulation Drug Combinations Interleukin 1 Receptor Antagonist Protein Endocrinology medicine.anatomical_structure Neuroprotective Agents chemistry Retinal ganglion cell Nerve Degeneration NMDA receptor sense organs Neurology (clinical) Halothane Developmental Biology medicine.drug Interleukin-1 |
Zdroj: | Brain research. 910(1-2) |
ISSN: | 0006-8993 |
Popis: | In this study we determine if interleukin-1beta (IL-1beta) modulates N-methyl-D-aspartate (NMDA)-induced retinal damage. Sprague-Dawley rats were anesthetized with inhalation of halothane, after which a single injection of 5 microl of IL-1beta (0.1 to 10 ng/eye) (and/or IL-1 receptor antagonist (IL-1ra)) for experimental eyes was administered. Two days later (or simultaneously), NMDA (20 nmol) was injected into the vitreous space. One week later, each eye was enucleated and transverse sections were subjected to morphometric analysis. Enzyme-linked immunosorbent assay (ELISA) was conducted for the determination of IL-1beta levels in retina. Immunohistochemical and immunoblot studies were also performed. In eyes that received an intravitreal injection of IL-1beta (0.1 to 10 ng/eye), significant thinning of the inner plexiform layer (IPL) was observed (P |
Databáze: | OpenAIRE |
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