Portable and sensitive Ag nanorods based SERS platform for rapid HIV-1 detection and tropism determination
| Autor: | Jitendra P. Singh, Dipen Desai, Sneha Senapati, Yadav Sp, Smita Kulkarni, Shashank K. Gahlaut |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Silver
Human immunodeficiency virus (HIV) Early detection HIV Infections 02 engineering and technology medicine.disease_cause Spectrum Analysis Raman 01 natural sciences Tropism Virus Colloid and Surface Chemistry 0103 physical sciences medicine Humans Physical and Theoretical Chemistry Chromatography Nanotubes 010304 chemical physics Chemistry Substrate (chemistry) Surfaces and Interfaces General Medicine Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology HIV-1 Nanorod 0210 nano-technology Silver nanorods Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 198 |
| ISSN: | 1873-4367 |
| Popis: | In this study, surface-enhanced Raman scattering (SERS) based field-deployable platform has been explored for early detection and distinction of the human immunodeficiency virus (HIV-1). A highly optimized silver nanorods array, fabricated using glancing angle deposition technique was used as SERS substrate. Distinct signature peaks for varying concentrations (102 to 106 copies/mL) were identified in five different HIV-1 subtypes (A, B, C, D, and CRF02_AG). Binding of viruses directly with Ag nanorods without using antibodies or intermediate reagents is shown. The purified viruses were spiked in water and healthy plasma to capture pure HIV-1 peaks. Distinct peaks were also captured for the X4 and R5 tropic strains suggesting tropism based detection. The above data was further confirmed and analyzed statistically using a multivariate tool. Thus, the present study indicates the ability of the SERS platform to detect and differentiate the HIV-1 virus implying its further validation using clinical specimens and isolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect