Alterations in cerebral metabolomics and proteomic expression during sepsis
| Autor: | Jochen Hinkelbein, Martin H. Maurer, Armin Kalenka, Robert E. Feldmann, Dominik W. Schelshorn, Charlotte Schubert, Anna Peterka |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Proteomics medicine.medical_treatment Nerve Tissue Proteins Biology Pharmacology Sepsis Cellular and Molecular Neuroscience Metabolomics Developmental Neuroscience medicine Escherichia coli Image Processing Computer-Assisted Animals Electrophoresis Gel Two-Dimensional Rats Wistar Ligature Cecum Gel electrophoresis Brain Chemistry Metabolism medicine.disease Rats Cell stress Neurology Proteome Immunology Disease Progression Neural Networks Computer Energy Metabolism Signal Transduction |
| Zdroj: | Current neurovascular research. 4(4) |
| ISSN: | 1567-2026 |
| Popis: | The cause of brain dysfunction during sepsis and septic encephalopathy is still under ongoing research. Sepsis induced changes in cerebral protein expression may play a significant role in the understanding of septic encephalopathy. The aim of the present study was to explore cerebral proteome alterations in septic rats. Fifty-six male Wistar rats were randomly assigned to a sepsis group (coecal ligature and puncture, CLP) or a control group (sham). Surviving rats were killed 24 or 48 hours after surgery and whole-brain lysates were used for two-dimensional gel electrophoresis and subsequent protein identification. Differentially expressed proteins were identified by mass spectrometry. Using the Ingenuity Pathways Analysis (IPA) tool, the relationship and interaction between the identified proteins was analyzed. Mortality was 53 % in septic rats. No rat of the control group was lost. More than 1,100 spots per gel were discriminated of which 29 different proteins were significantly (2-fold, P |
| Databáze: | OpenAIRE |
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