Detection of Streptococcus mutans by PCR amplification of spaP gene
| Autor: | E. J. Fernandez, Komei Fukui, K Hirota, K. Nemoto, Takahito Ono, Fusao Ota |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Molecular Sequence Data Biology Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Immunoenzyme Techniques Streptococcus mutans Mice chemistry.chemical_compound Bacterial Proteins Antigen law Gene duplication Animals Humans Gene Polymerase chain reaction DNA Primers Antigens Bacterial Membrane Glycoproteins Base Sequence Antibodies Monoclonal General Medicine Streptococcaceae biology.organism_classification Molecular biology chemistry Genes Bacterial Antigens Surface Bacteria DNA |
| Zdroj: | Journal of Medical Microbiology. 41:231-235 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-41-4-231 |
| Popis: | Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the spaP gene, which encodes the surface protein antigen I/II of Streptococcus mutans. A DNA fragment of c. 192 bp was amplified from lysed S. mutans cells or isolated DNA. With S. mutans cells, the lower limit of detection was 4-40 cfu. With these primers, 13 reference and 50 clinical strains of S. mutans were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The spaP gene PCR has potential for the rapid diagnosis of S. mutans infections. |
| Databáze: | OpenAIRE |
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