Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir
| Autor: | Ruifeng Li, Jinyuan Sun, Yinglu Cui, Tong Zhu, Bian Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetic Vectors
Firmicutes Gene Expression 02 engineering and technology Cellulase Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Enzyme Stability Extremophile Oil and Gas Fields Amino Acid Sequence Cloning Molecular Cellulose Molecular Biology 030304 developmental biology Thermostability 0303 health sciences biology Organic solvent Thermophile Temperature Sequence Analysis DNA General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Pulp and paper industry Petroleum reservoir Enzyme Activation chemistry Biofuel Biofuels Fermentation Solvents biology.protein 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 159:622-629 |
| ISSN: | 0141-8130 |
| Popis: | The manufacture of biofuels from cellulose is regarded as one of practicable strategies to meet increasing energy demand and alleviate environmental issues. Cellulases, which play an important role in the production of second-generation biofuels, are expected to be highly thermostable, halostable and organic solvent-stable to adapt to the harsh conditions in practical application. Here we cloned and characterized a novel cellulase (MaCel) from Mahella australiensis 50-1 BON, an anaerobic thermophile isolated from an oil reservoir. MaCel exhibited excellent thermostability, halostability as well as organic solvent stability, and could be efficiently produced in a yield of 1.7 × 106 U/L in 15 h with inexpensive culture medium. These results indicate that MaCel may be a suitable candidate for industrial applications, illustrating the potential benefits of enzymes from oil reservoir extremophiles in the manufacture of biofuels. |
| Databáze: | OpenAIRE |
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