Activation of the Meiotic Divisions inDrosophilaOocytes
| Autor: | Andrea W. Page, Terry L. Orr-Weaver |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Meiotic cell cycle
Biology 03 medical and health sciences 0302 clinical medicine Meiosis Translational regulation Protein biosynthesis Animals Cycloheximide Molecular Biology Cells Cultured Metaphase 030304 developmental biology Anaphase Genetics Protein Synthesis Inhibitors 0303 health sciences Meiosis II Egg Proteins Cell Biology Chromatin Cell biology Chloramphenicol Protein Biosynthesis Oocytes Metaphase i Drosophila Colchicine 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. (2):195-207 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1997.8506 |
| Popis: | Key meiotic events in many organisms are controlled at the translational level. In this study, we examine the role of translational regulation in the meiotic cell cycle of Drosophila. In order to address this question, we developed a system for activating Drosophila oocytes in vitro. With this method, hundreds of mature oocytes can be activated to resume and complete meiosis. The stages of meiosis are normal by cytological criteria, and the timing of the meiotic divisions is similar to that of eggs activated in vivo. We use this system to examine the role of protein synthesis in regulating the progression of meiosis and the maintenance of the metaphase I arrest. We find that synthesis of new proteins after metaphase I is not required for anaphase I, meiosis II, or the decondensation of the meiotic products. Also, continued protein synthesis is not required to maintain the metaphase I arrest. New protein synthesis is required, however, for proper chromatin recondensation after meiosis. |
| Databáze: | OpenAIRE |
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