Prior to extension, Transcriptomes of fibroblast-like Synoviocytes from extended and Polyarticular juvenile idiopathic arthritis are indistinguishable

Autor: Carlos D. Rose, Megan M. Simonds, AnneMarie C. Brescia, Kathleen E. Sullivan, Suzanne M. McCahan
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
lcsh:Diseases of the musculoskeletal system
Microarray
Cell Culture Techniques
Arthritis
Transcriptome
0302 clinical medicine
Synovial Fluid
Gene expression
Immunology and Allergy
Medicine
Child
skin and connective tissue diseases
lcsh:RJ1-570
Synoviocytes
JIA subtypes
Child
Preschool
Disease Progression
Female
Research Article
musculoskeletal diseases
medicine.medical_specialty
Adolescent
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Rheumatology
Internal medicine
Humans
Synovial fluid
Biomarkers
business.industry
Microarray analysis techniques
Extended JIA
lcsh:Pediatrics
Juvenile idiopathic arthritis
Microarray Analysis
medicine.disease
Fibroblast-like synoviocytes
Arthritis
Juvenile
030104 developmental biology
Pediatrics
Perinatology and Child Health
Immunology
Oligoarticular Juvenile Idiopathic Arthritis
lcsh:RC925-935
business
030215 immunology
Zdroj: Pediatric Rheumatology Online Journal, Vol 16, Iss 1, Pp 1-7 (2018)
Pediatric Rheumatology Online Journal
ISSN: 1546-0096
DOI: 10.1186/s12969-017-0217-6
Popis: Background Our intent was to identify differences between the transcriptome of fibroblast-like synoviocytes (FLS) in oligoarticular juvenile idiopathic arthritis (JIA) before extension when compared to persistent subtype of JIA, when the two are clinically indistinguishable. Additionally, we sought to determine if differences between the transcriptomes of FLS from extended-to-be and polyarticular course JIA could be detected. Our hypothesis was that intrinsic differences in the transcriptome of the FLS from extended-to-be JIA would distinguish them from persistent oligoarticular JIA, before the course is clinically apparent. Methods Global gene expression was defined in cultured FLS from 6 controls, 12 JIA with persistent course, 7 JIA prior to extension (extended-to-be), 4 JIA with extended course and 6 polyarticular onset, using Affymetrix Human GeneChips 133plus2.0. Results Bioconductor Linear Models for Microarray Analysis revealed 22 probesets with differential expression between persistent and extended-to-be FLS at 15% FDR, however only 2 probesets distinguished extended-to-be from extended and none distinguished extended-to-be and polyarticular at 15% FDR. Differences in extended and polyarticular gene expression profiles were not detected. Confirmation of select genes was done on the RNA level by RT-qPCR and on the protein level in synovial fluid by ELISA. Conclusions The transcriptome of FLS from extended-to-be juvenile idiopathic arthritis is distinct from persistent course before a clinical distinction can be made. Additionally, the transcriptome of extended-to-be and polyarticular course, including those who have already extended, are indistinguishable. These gene expression data suggest that FLS already reflect a polyarticular behavior early in disease course, suggesting that extended-to-be may be “latent polyarticular” at onset. These differences can be used to develop early biomarkers of disease course, allowing for better-informed treatment decisions. Electronic supplementary material The online version of this article (10.1186/s12969-017-0217-6) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje