Asynchronous lineage priming determines commitment to T cell and B cell lineages in fetal liver

Autor: Ana Cumano, Rachel Golub, Paulo Vieira, Guillaume Soubigou, Pablo Pereira, Claire Berthault, Sylvestre Chea, Odile Burlen-Defranoux, Cyrille Ramond
Přispěvatelé: Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, Lymphopoïèse (Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC), Transcriptome et Epigénome (PF2), Institut Pasteur [Paris] (IP), Supported by the Pasteur Institute, Institut National de la Santé et de la Recherche Médicale (INSERM), the Ministère de la Recherche (C.B., C.R. and S.C.), Association pour la Recherche sur le Cancer (S.C. and R.G.), La Ligue Contre Le Cancer (C.B. and C.R.), Université Paris Diderot (C.B., R.G. and S.C.), Université Pierre et Marie Curie (C.R.), the Agence Nationale de la Recherche (project Myeloten (R.G.), program REVIVE (Investment for the Future) (A.C.), and project Twothyme (A.C.) and the Pasteur-Weizmann Foundation (A.C.), We thank A. Bandeira and D. Guy-Grand for critical reading, S. Novault and S. Schmutz for support, H.-R. Rodewald (German Cancer Center DKFZ) for the IL-7Rα-Cre mouse line, and P. Chambon (IGBMC Strasbourg) for the TSLP-KO mice. This work benefited from data assembled by the ImmGen Consortium, ANR-13-ISV1-0003,MYELOTEN,DEREGULATION DE L'HEMATOPOIESE PAR LA SUREXPRESSION DE L'INTERLEUKINE-10 : IMPLICATIONS DANS LE DEVELOPPEMENT DE PATHOLOGIES HEMATOLOGIQUES(2013), ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-14-CE11-0022,Twothyme,Deux progéniteurs hématopoïétiques différents établissent le compartiment de lymphocytes T: tester un nouveau paradigme du développement T.(2014), PRES Sorbonne Paris Cité-Université Paris Diderot - Paris 7 (UPD7), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], ANR-10-LABX-0073/10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010)
Rok vydání: 2017
Předmět:
Zdroj: Nature Immunology
Nature Immunology, 2017, Epub ahead of print, ⟨10.1038/ni.3820⟩
Nature Immunology, Nature Publishing Group, 2017, ⟨10.1038/ni.3820⟩
ISSN: 1529-2916
1529-2908
DOI: 10.1038/ni.3820
Popis: International audience; The molecular events that initiate lymphoid-lineage specification remain unidentified because the stages of differentiation during which lineage commitment occurs are difficult to characterize. We isolated fetal liver progenitor cells undergoing restriction of their differentiation potential toward the T cell-innate lymphoid cell lineage or the B cell lineage. Transcripts that defined the molecular signatures of these two subsets were sequentially upregulated in lympho-myeloid precursor cells and in common lymphoid progenitor cells, respectively, and this preceded lineage restriction; this indicates that T cell-versus-B cell commitment is not a binary fate 'decision'. The T cell-bias and B cell-bias transcriptional programs were frequently co-expressed in common lymphoid progenitor cells and were segregated in subsets biased toward T cell differentiation or B cell differentiation, after interleukin 7 (IL-7) signaling that controlled the number of progenitor cells engaging in T cell differentiation versus B cell differentiation.
Databáze: OpenAIRE
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