Tumor necrosis factor alpha (TNFalpha) induces pro-matrix metalloproteinase 9 production in human uterine cervical fibroblasts but interleukin 1alpha antagonizes the inductive effect of TNFalpha
| Autor: | Hideaki Nagase, Akira Ito, Yutaka Ogata, Yo Mori, Takashi Sato |
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| Rok vydání: | 1996 |
| Předmět: |
Biophysics
Cervix Uteri Matrix metalloproteinase 12-O-Tetradecanoylphorbol-13-acetate Biochemistry chemistry.chemical_compound Alkaloids Interleukin 1α Structural Biology Genetics Tumor necrosis factor α Humans Collagenases Enzyme Inhibitors Molecular Biology Polyacrylamide gel electrophoresis Protein kinase C Cells Cultured Protein Kinase C Enzyme Precursors Chemistry Tumor Necrosis Factor-alpha Interleukin Human uterine cervical fibroblast Matrix metalloproteinase 9 Cell Biology Fibroblasts Staurosporine Molecular biology Matrix Metalloproteinase 9 Enzyme Induction Progelatinase B Tetradecanoylphorbol Acetate Tumor necrosis factor alpha Female Interleukin-1 |
| Zdroj: | FEBS letters. 392(2) |
| ISSN: | 0014-5793 |
| Popis: | We have examined the regulation of precursor of matrix metalloproteinase 9 (proMMP-9)/progelatinase B production by tumor necrosis factor alpha (TNFalpha) and interleukin 1alpha (IL-1alpha) using human uterine cervical fibroblasts. TNFalpha, but not IL-1alpha, induces the production of proMMP-9 in the cervical cells. IL-alpha, however, suppresses the TNFalpha-induced proMMP-9 production. 12-0-tetradecanoylphorbol 13-acetate (TPA) also stimulates the cervical cells to produce proMMP-9, and IL-1alpha synergistically enhances its production. TNFalpha-induced proMMP-9 production is not mediated by protein kinase C (PKC), whereas the effect of IL-1alpha is through PKC. By contrast, proMMP-3/prostromelysin 1 is up-regulated by TNFalpha or TPA in the presence of IL-1alpha, whose modulation is PKC-dependent. The suppressive effect of IL-1alpha on the TNFalpha-induced proMMP-9 production is a new biological effect of IL-1 on MMP production. |
| Databáze: | OpenAIRE |
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