Fluorescent flavonoids for endoplasmic reticulum cell imaging†
| Autor: | Kyle T. Whiddon, Qin Liu, Leah P. Shriver, Alexandra Taraboletti, Michael C. Konopka, Lucas McDonald, Bin Liu, Yi Pang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Molecular mass
Biocompatibility 010405 organic chemistry Endoplasmic reticulum Biomedical Engineering Substituent General Chemistry General Medicine Biology 010402 general chemistry 01 natural sciences Fluorescence Article 0104 chemical sciences chemistry.chemical_compound Biochemistry chemistry In vivo Organelle Protein biosynthesis General Materials Science |
| Popis: | Visualization of subcellular organelles in vivo is critical for basic biomedical research and clinical applications. Two new flavonoids with an amide substituent were synthesized and characterized. The flavonoids were nearly non-fluorescent in aqueous environment, but exhibited two emission peaks (one λem at 495-536 nm and the other at 570-587 nm) in organic solvents, which were assigned to the excited normal (N*) and tautomer (T*) emission. When the dyes were examined on oligodendrocyte cells, they were found to selectively accumulate in the endoplasmic reticulum (ER), a eukaryotic organelle involved in lipid and protein synthesis, giving fluorescence turn-on. The ER-selective flavonoids could be a valuable tool due to its low molecular mass ( |
| Databáze: | OpenAIRE |
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