SPME-GC/MS Analysis of Methanol in Biospecimen by Derivatization with Pyran Compound
| Autor: | Yong Ae Jeong, Iel Soo Bang, Dae Jun Ahn, Joon-Bae Lee |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pharmaceutical Science
Solid-phase microextraction 01 natural sciences Gas Chromatography-Mass Spectrometry Article Analytical Chemistry gas chromatography/mass spectrometry 03 medical and health sciences chemistry.chemical_compound Drug Discovery derivatization Humans Formate Physical and Theoretical Chemistry Derivatization Pyrans 0303 health sciences Chromatography Chemistry Methanol 030302 biochemistry & molecular biology 010401 analytical chemistry Organic Chemistry Benzenesulfonates 0104 chemical sciences Methanol poisoning Chemistry (miscellaneous) Standard addition solid-phase microextraction Molecular Medicine Gas chromatography Gas chromatography–mass spectrometry |
| Zdroj: | Molecules Volume 25 Issue 1 |
| ISSN: | 1420-3049 |
| Popis: | Methanol is metabolized in the body to highly toxic formaldehyde and formate when consumed accidentally. Methanol has been typically analyzed with gas chromatography-flame ionization detector (GC-FID). However, its retention time may overlap with other volatile compounds and lead to confusion. Alternative analysis of methanol using gas chromatography/mass spectrometry (GC/MS) also has limitations due to its similar molecular weight with oxygen and low boiling point. In this study, methanol and internal standard of deuterium-substituted ethanol were derivatized with 3,4-dihydro-2H-pyran under acid catalysis using concentrated hydrochloric acid. The reaction products including 2-methoxytetrahydropyran were extracted with solid-phase microextraction followed by GC/MS analysis. This method was successfully applied to measure the lethal concentration of methanol in the blood of a victim with a standard addition method to overcome the complex matrix effect of the biospecimen. Identification of the metabolite formate by ion chromatography confirmed the death cause to be methanol poisoning. This new method was a much more convenient and reliable process to measure methanol in complex matrix samples by reducing sample pretreatment effort and cost. |
| Databáze: | OpenAIRE |
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