New facet of non-O1/non-O139 Vibrio cholerae hemolysin A: a competitive factor in the ecological niche
| Autor: | Onrapak Reamtong, Pornphan Diraphat, Pichet Ruenchit, Wanpen Chaicumpa, Kanokrat Siripanichgon |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Biology Hemolysin Proteins medicine.disease_cause Applied Microbiology and Biotechnology Microbiology El Tor 03 medical and health sciences fluids and secretions Bacterial Proteins Rivers medicine Vibrio cholerae Type VI secretion system Ecological niche Type I Secretion Systems Ecology Strain (chemistry) Hemolysin biochemical phenomena metabolism and nutrition Thailand bacterial infections and mycoses biology.organism_classification 030104 developmental biology Microbial Interactions bacteria Bacterial inhibition assay |
| Zdroj: | FEMS Microbiology Ecology. 94 |
| ISSN: | 1574-6941 |
| DOI: | 10.1093/femsec/fix113 |
| Popis: | Different serogroups of Vibrio cholerae may inhabit the same ecological niche. However, serogroup O1/O139 strains are rarely isolated from their ecological sources. Quite plausibly, the non-O1/non-O139 vibrios and other bacterial species suppress growth of O1/O139 strains that share the same niche. Our bacterial inhibition assay data indicated that certain non-O1/non-O139 strains used a contact-dependent type VI secretion system (T6SS) to suppress growth of the O1 El Tor, N16961 pandemic strain. Comparative proteomics of the O1 and the suppressive non-O1/non-O139 strains co-cultured in a simulated natural aquatic microcosm showed that SecB and HlyD were upregulated in the latter. The HlyD-related effective factor was subsequently found to be hemolysin A (HlyA). However, not all hlyA-positive non-O1/non-O139 strains mediated growth suppression of the N16961 V. cholerae; only strains harboring intact cluster I HlyA could exert this activity. The key feature of the HlyA is located in the ricin-like lectin domain (β-trefoil) that plays an important role in target cell binding. In conclusion, the results of this study indicated that non-O1/non-O139 V. cholerae suppressed the growth of the O1 pandemic strain by using contact-dependent T6SS as well as by secreting the O1-detrimental hemolysin A during their co-persistence in the aquatic habitat. |
| Databáze: | OpenAIRE |
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