Cerebral inoculation of human A53T α-synuclein reduces spatial memory decline and amyloid-β aggregation in APP/PS1 transgenic mice of Alzheimer’s disease
| Autor: | Jin-Shun Qi, Yi-ning Hao, Zhao-Jun Wang, Yu-hao Zhai, Hao-yue Wang, Meng-Ming Hu, Ze-wen Wu, Biao Yang, Qi-xuan Lu, Mei-Na Wu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Genetically modified mouse medicine.medical_specialty animal diseases Transgene Morris water navigation task Mice Transgenic Plaque Amyloid Biology Fibril Protein Aggregation Pathological Amyloid beta-Protein Precursor Mice 03 medical and health sciences 0302 clinical medicine Western blot Alzheimer Disease Internal medicine mental disorders Presenilin-1 medicine Animals Humans Cognitive Dysfunction Senile plaques Maze Learning Spatial Memory Memory Disorders Amyloid beta-Peptides medicine.diagnostic_test General Neuroscience Wild type Brain nervous system diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology nervous system alpha-Synuclein Immunohistochemistry Cognition Disorders 030217 neurology & neurosurgery |
| Zdroj: | Brain Research Bulletin. 143:116-122 |
| ISSN: | 0361-9230 |
| Popis: | Amyloid-β (Aβ) peptide and α-synuclein (α-syn) are major components of senile plaques in Alzheimer's disease (AD) and Lewy bodies in Parkinson's disease (PD), respectively. Co-occurrence of Aβ and α-syn in the senile brains of AD and LB diseases suggests interactions between the two proteins. However, the significance of the overlapping deposition, especially the effects of α-syn on the Aβ aggregation, still remains to be clarified. In the present study, we investigated the effects of α-syn pre-formed fibrils (PFFs) injection on the cognitive behaviors and Aβ deposition in the brain of APP/PS1 transgenic AD mice by using Morris water maze (MWM) test, immunohistochemistry and western blot techniques. We found that APP/PS1 transgenic mice exhibited an obvious elevation in the α-syn load, as well as Aβ deposition in the brain compared with wild type of C57 BL littermates. 5 months after cerebral injection of exogenous α-syn, MWM tests showed an alleviation in cognitive impairments in APP/PS1 mice; western blot and immunohistochemistry experiments also exhibited a significant reduction in Aβ level in the brain of APP/PS1 mice injected with α-syn. These results suggest that α-syn aggregated in the brain of AD may act as a protective factor and defend the brain tissue from early Aβ deposition and cognitive deficits. |
| Databáze: | OpenAIRE |
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