Sequence Analysis of the HN Gene of Parainfluenza Virus Type 2
| Autor: | J. A. Southern, Richard E. Randall, B. Precious |
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| Rok vydání: | 1990 |
| Předmět: |
Genes
Viral Paramyxoviridae Sequence analysis viruses Molecular Sequence Data Polymerase Chain Reaction Respirovirus Virus Parainfluenza virus type 2 Sequence Homology Nucleic Acid Virology Complementary DNA Animals Amino Acid Sequence RNA Messenger HN Protein Vero Cells Gene Library Base Sequence biology cDNA library Nucleic acid sequence DNA biology.organism_classification Molecular biology Parainfluenza Virus 2 Human Mumps virus RNA Viral |
| Zdroj: | Journal of General Virology. 71:1163-1168 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-71-5-1163 |
| Popis: | A cDNA library was constructed in lambda gt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with 32P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin-neuraminidase (HN) gene were identified by sequence comparisons with known parainfluenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43% amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses. |
| Databáze: | OpenAIRE |
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