Investigation of P1/HC-Pro-Mediated ABA/Calcium Signaling Responses via Gene Silencing through High- and Low-Throughput RNA-seq Approaches
| Autor: | Zhao-Jun Pan, Chien-Kang Huang, Thanh Ha Pham, Yu-Ling Hung, Qian-Wen Shang, Hsin-Ping Wang, Yen-Hsin Chiu, Wei-Lun Wei, Shih-Shun Lin |
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| Rok vydání: | 2021 |
| Předmět: |
Arabidopsis
Biology Microbiology Article Transcriptome chemistry.chemical_compound Gene Expression Regulation Plant Virology Gene silencing Turnip mosaic virus Calcium Signaling Gene Abscisic acid Calcium signaling P1/HC-ProTu Jasmonic acid fungi food and beverages stress response HTP-Seq biology.organism_classification Plants Genetically Modified QR1-502 LTP-Seq Cell biology RNA silencing Infectious Diseases chemistry ABA signaling calcium signaling RNA Interference |
| Zdroj: | Viruses Viruses; Volume 13; Issue 12; Pages: 2349 Viruses, Vol 13, Iss 2349, p 2349 (2021) |
| ISSN: | 1999-4915 |
| Popis: | The P1/HC-Pro viral suppressor of potyvirus suppresses posttranscriptional gene silencing (PTGS). The fusion protein of P1/HC-Pro can be cleaved into P1 and HC-Pro through the P1 self-cleavage activity, and P1 is necessary and sufficient to enhance PTGS suppression of HC-Pro. To address the modulation of gene regulatory relationships induced by turnip mosaic virus (TuMV) P1/HC-Pro (P1/HC-ProTu), a comparative transcriptome analysis of three types of transgenic plants (P1Tu, HC-ProTu, and P1/HC-ProTu) were conducted using both high-throughput (HTP) and low-throughput (LTP) RNA-Seq strategies. The results showed that P1/HC-ProTu disturbed the endogenous abscisic acid (ABA) accumulation and genes in the signaling pathway. Additionally, the integrated responses of stress-related genes, in particular to drought stress, cold stress, senescence, and stomatal dynamics, altered the expressions by the ABA/calcium signaling. Crosstalk among the ABA, jasmonic acid, and salicylic acid pathways might simultaneously modulate the stress responses triggered by P1/HC-ProTu. Furthermore, the LTP network analysis revealed crucial genes in common with those identified by the HTP network in this study, demonstrating the effectiveness of the miniaturization of the HTP profile. Overall, our findings indicate that P1/HC-ProTu-mediated suppression in RNA silencing altered the ABA/calcium signaling and a wide range of stress responses. |
| Databáze: | OpenAIRE |
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