Quantification of cell-free circulating mitochondrial DNA copy number variation in hepatocellular carcinoma
| Autor: | Burhanettin Yalçınkaya, Didem Tastekin, Fatih Güzelbulut, Muslum Akgoz, Sadrettin Pençe |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Revista da Associação Médica Brasileira, Volume: 68, Issue: 9, Pages: 1161-1165, Published: 07 OCT 2022 Revista da Associação Médica Brasileira v.68 n.9 2022 Revista da Associação Médica Brasileira Associação Médica Brasileira (AMB) instacron:AMB |
| ISSN: | 1806-9282 0104-4230 |
| DOI: | 10.1590/1806-9282.20210368 |
| Popis: | SUMMARY OBJECTIVE: Hepatocellular carcinoma is the most common primary malignant liver tumor. Mitochondrial DNA copy number has been shown to be associated with various malignancies. However, there has not been any study on the absolute quantification of mtDNA copy number in hepatocellular carcinoma. The aim of this study was to develop a new method for absolute quantification of mtDNA copy number and to relatively quantify the variations in the mtDNA copy number in hepatocellular carcinoma patients in comparison with healthy individuals. METHODS: Venous blood samples were collected from both hepatocellular carcinoma patients (34) and healthy individuals (34). Circulating cell-free DNAs were isolated and the relative quantification of mtDNA copy number variation was determined using quantitative polymerase chain reaction and digital polymerase chain reaction. RESULTS: It was found that the relative mtDNA copy number was significantly decreased in hepatocellular carcinoma patients in comparison with the control group (p |
| Databáze: | OpenAIRE |
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