Determination of Acitretin in the Skin, in the Suction Blister, and in Plasma of Human Volunteers after Multiple Oral Dosing
| Autor: | Hot Bun, Jean-Philippe Laugier, Howard I. Maibach, J.-M. Geiger, Christian Surber, Alain Durand, Klaus-Peter Wilhelm |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Biopsy Administration Oral Pharmaceutical Science Sensitivity and Specificity High-performance liquid chromatography Drug Administration Schedule Acitretin Blister Pharmacokinetics Oral administration Blood plasma medicine Humans Shave biopsy Chromatography High Pressure Liquid Skin Detection limit Chromatography integumentary system medicine.diagnostic_test Chemistry Exudates and Transudates Middle Aged Suction blister medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 83:623-628 |
| ISSN: | 0022-3549 |
| Popis: | Several HPLC methods for quantification of acitretin and its 13‐cis isomer in biological fluids have been described. Only limited data are available on determination of this drug in skin samples. Our objective was to improve the sensitivity and selectivity of existing methods to measure drug in small skin samples from humans treated with acitretin. With a new optimized mobile phase [methanol: acetonitrile (7:3, v/v), purified water with 1.5% (v/v) acetic acid, mixed in a 85:15 ratio (v/v)] and a new internal standard (arotinoid ethyl sulfone), a limit of quantification of 1 ng/g tissue was reached. Nine male volunteers were given an oral daily dose of 50 mg acitretin for up to 28 days. Blood and skin samples (punch and shave biopsies, suction blister skin, and fluid) were taken at various time points during and after treatment. Drug concentration and metabolism in plasma and skin samples appeared to be linked in that the trans‐isomer concentration was always higher than the cis‐isomer concentration during dosing and 3 h after the last dose. However, 7 and 14 days after the last dose in plasma and in all tissue samples (except the shave biopsy), the all‐trans‐acitretin concentration rapidly decreased and approached the detection limit. In the shave biopsy, the all‐trans‐acitretin concentration remained higher than the 13‐cis‐acitretin concentration. Furthermore, the elimination of two isomers from the shave biopsy was delayed. Our HPLC method has provided a suitable tool for pharmacokinetic and drug monitoring studies of all‐trans‐acitretin and 13‐cis‐acitretin that can be performed by any laboratory with a darkroom and a basic isocratic HPLC system. |
| Databáze: | OpenAIRE |
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