Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis
| Autor: | Susan M. Lunte, Manjula B. Wijesinghe, Kelci M. Schilly, Shamal M. Gunawardhana |
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| Rok vydání: | 2020 |
| Předmět: |
Microdialysis
02 engineering and technology 01 natural sciences Biochemistry Article Analytical Chemistry law.invention Electrophoresis Microchip Mice In vivo law Animals Electrodes Chemiluminescence Sheep Chromatography Chemistry 010401 analytical chemistry Substrate (chemistry) Electrochemical Techniques Equipment Design 021001 nanoscience & nanotechnology Fluorescence Amperometry 0104 chemical sciences Electrophoresis Electrode 0210 nano-technology |
| Zdroj: | Anal Bioanal Chem |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-020-02647-z |
| Popis: | Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided. Graphical abstract. |
| Databáze: | OpenAIRE |
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