Simultaneous determination of two antioxidants, uric and ascorbic acid, in animal tissue by high-performance liquid chromatography
| Autor: | R. Pérez-Campo, G. Barja de Quiroga, Mónica López-Torres, C. Rojas |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Biophysics Ascorbic Acid Biochemistry High-performance liquid chromatography Antioxidants Mice chemistry.chemical_compound Drug Stability Liver tissue medicine Animals Molecular Biology Chromatography High Pressure Liquid Reproducibility Chromatography Cell Biology Ascorbic acid Sample stability Uric Acid Liver chemistry Uric acid Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Analytical Biochemistry. 199:81-85 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(91)90272-u |
| Popis: | A rapid and simple method for the simultaneous analysis of uric and ascorbic acid in extracts of animal tissue is described. The method uses reversed-phase ion-pair chromatography with ultraviolet detection. The technique allows efficient separation of both acids while showing high selectivity, recovery, reproducibility, and sample stability. Calculated levels of both substances in mouse liver tissue were 1.00 ± 0.05 μmol ascorbic acid/g and 130 ± 5 nmol uric acid/g. |
| Databáze: | OpenAIRE |
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